mLIF
LIF promotes long-term maintenance of mouse pluripotent stem cells. LIF is also involved in a number of other biological processes including neural and hematopoietic cell differentiation. *For sale internationally only.
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| Recommended use | Culturing of mouse ES or iPS cells |
| Product Unit | Vial |
| Size | 100 µg |
| Storage | Store refrigerated at 2-8°C |
| PluriQ | Tested on human and mouse pluripotent stem cells over multiple passages |
| Quality Testing | Purity, activity and sterility |
| Shipping | Room temperature |
| Other | Activity: 5ng = 1000 Units |
mLIF
Leukemia Inhibitory Factor (LIF) is a lymphoid factor which is involved in a number of reproductive, inflammatory, developmental and regenerative processes. LIF promotes long-term maintenance of pluripotent stem cells by suppressing spontaneous differentiation. LIF is also involved in a number of other biological processes including neural and hematopoietic cell differentiation, bone and fat metabolism, and mitogenesis of certain factor dependent cell lines.
Applications for Mouse pluripotent (iPS/ES) stem cell culturing
Added as a supplement to cell culture medium to promote long-term maintenance and propagation of undifferentiated mouse embryonic and induced pluripotent stem cells.
Product Formulation and Quality testing
- Formulation: Lyophilized in PBS, pH 7.2, sterile filtered. Carrier-free.
- Identity: Confirmed by western blot analysis using protein specific antibody.
- Activity: Bioactivity is assayed using murine M1 myeloid leukemia cells and mouse ES cells. GlobalStem mLIF has an equivalent activity to 1000 U/ml when used at a concentration of only 5 ng/ml.
- Purity: > 95%, by SDS-PAGE
- Free of Contaminants: Confirmed that endotoxin levels are less than 0.005ng/μg product (0.05EU/μg) as measured by turbidimetric kinetic assay. This level is 20 times lower than that of competitors’ products.
- Tested to confirm the product carries no pyrogenic or pro-inflammatory contaminants, as assayed with monocyte activation test (MAT assay) using Human 10-plex Cytokine Assay measuring IL-6, TNF-alpha and IL-1beta induction.
- Sterility is confirmed and product is mycoplasma-free
Activity Graph: Proliferation assay measuring ability of GlobalStem mLIF to induce differentiation of mouse M1 myeloid leukemia cells.
ED50 = 0.05 ng/ml.
