Primary Rat Neural Stem Cells
Rat Neural Stem Cells from Sprague-Dawley E14 cortex are ready for use. They are multipotent and delivered at passage number zero (P0) and are ready for expansion or differentiation into neurons, astrocytes, and oligodendrocytes.
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| Recommended use | Neural differentiation |
| Type | Rat Sprague-Dawley E14 cortex |
| Product Unit | Cryopreserved vial |
| Size | 1 vial, >1 million cells |
| Storage | Liquid nitrogen |
| Quality Testing | Tested for healthy undifferentiated growth, Sterility, Mycoplasma, Post thaw viability |
| Shipping | Dry ice |
Primary neural stem cells isolated from the cortical neuroepithelium of the fetal rat (embryonic day 14), P0. They are reproducible and multipotent. After plating, the cells are ready for expansion or differentiation into neurons, astrocytes and oligodendrocytes. They can be transfected, transplanted or differentiated into electrically active neurons. We take care of everything from dissection and isolation to cryopreservation. Plus, we perform extensive quality control and performance testing on every batch.
- Doubling time: 18 hours
- Medium renewal: Every two days add 10 ng/mL bFGF (GSR-2001) every day.
- Complete growth medium: N2/DMEM-F12* plus 10 ng/mL bFGF (GSR-2001).
- Subculture frequency: Every 3–4 days
- Cryopreservation medium: N2/DMEM-F12* plus 10% DMSO.
Undirected differentiation to neurons, astrocytes and oligodendrocytes can be achieved by the withdrawal of the mitogen basic fibroblast growth factor (bFGF).
Figure 1. Differentiated Rat Neural Stem Cells, co-stained for the neuronal marker, MAP2(red), and the astrocyte marker, GFAP (green). The nuclei are stained with DAPI (blue).
Figure 2. Differentiated Rat Neural Stem Cells, co-stained for the neuronal marker, Tuß3(left, green), and the astrocyte marker, GFAP (right, green). The nuclei in both images are stained with DAPI (blue).