Human Fibroblasts 4M untreated D12
Our human fibroblasts are isolated from newborn foreskin (Donor 12). They are ideal feeder cells for supporting healthy undifferentiated human ES and iPS cells including support during reprogramming. Untreated.
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| Recommended Use | Maintenance of undifferentiated ESC and iPSC lines |
| Cell Source | Human Foreskin |
| Product Unit | Cryopreserved Vial |
| Size | 4-5 million cells/vial |
| Treatment | Untreated |
| Storage | Vapor phase of liquid nitrogen |
| Quality Testing | Tested for high efficient maintenance of human pluripotent cells, sterility, human pathogens, mycoplasma, post thaw viability |
| Shipping | Dry Ice |
| Passage Number | P9 |
| Other | Donor 12 |
NuFF: Newborn Human Foreskin Fibroblasts
Quality human feeder cells for human pluripotent stem cells
The success of maintaining human ESC and iPSC begins with the propagation of healthy feeder cultures. We recommend using our human newborn fibroblasts (NuFF) to support your human pluripotent cell lines. The NuFF feeder cells support and maintain human ESC and iPSC in the undifferentiated state. Our NuFF have been rendered mitotically inactive via irradiation or chemical treatment with Mitomycin C. Untreated NuFF are also available.
Meticulous Quality Control
Work with confidence. Comprehensive testing of each lot minimizes contamination risks to your lab. Our NuFF are derived and comprehensively tested on human ES cells to ensure robust and consistent performance with every lot. In addition they are:
- Negative for bacterial/fungal contamination
- Negative for Mycoplasma – PCR assay
- Negative for human pathogens
- Post thaw viability: typically greater than 95%
Recommended Plating
The recommended seeding density is 2 x 104 to 3 x 104 cells/cm2; however, please follow the culture instructions provided with the specific ESC or iPSC lines that you are using. These cells should be plated 24 hours prior to plating the ES cells and can be used for 7–10 days. If the feeder cells are too sparse, they may not maintain the pluripotent cells without differentiation, and the pluripotent cells may not attach well. If the MEF are too dense, the feeder layer may detach from the plate, and the culture will be lost.
Save Time and Money
Save time and trouble dealing with an animal facility, performing tedious and time-consuming dissections and the time required to expand and test the cells. Day after day – week after week. Focus on your research.
Safe handling
Work with confidence. Our comprehensive contamination tests include human pathogen and Mycoplasma detection. This significantly minimizes the threat of contamination in your lab allowing you to work with greater confidence.