CF-1 MEF 4M IRR
CF-1 MEFs are ideal for supporting healthy undifferentiated human and mouse ES and iPS cells in culture. CF-1 MEFs are the traditional feeder cells for culturing pluripotent cells. They are mitotically arrested by irradiation treatment and will not proliferate.
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| Recommended Use | Maintenance of undifferentiated ESC and iPSC lines |
| Cell Source | Murine CF-1 |
| Product Unit | Cryopreserved Vial |
| Size | 4-5 million cells/vial |
| Treatment | Irradiation |
| Storage | Vapor phase of liquid nitrogen |
| Quality Testing | Tested for high efficient maintenance of mouse and human pluripotent cells, Sterility, mouse pathogens, Mycoplasma, Post thaw viability |
| Shipping | Dry Ice |
| Passage Number | P3 |
Quality MEFs for Maintaining Quality Pluripotent Cells
The success of maintaining human and mouse ESC/iPSC begins with the propagation of healthy feeder cultures. We recommend using our mouse embryonic fibroblasts (MEF) as feeders. The MEF feeder cells support and maintain ESC/iPSC in the undifferentiated state. Our MEF have been rendered mitotically inactive via irradiation or chemical treatment with Mitomycin C. Untreated MEF are also available.
Our MEF have successfully been used with ESC/iPSC lines from species other than human or mouse. If you want to use them with pluripotent stem cells derived from other species please test them first to ensure compatibility.
Save Time and Money
Save time and trouble dealing with an animal facility, performing tedious, time-consuming dissections, and the time required to expand and test the cells - day after day, week after week. Focus on your research.
Meticulous Quality Control
Work with confidence. Comprehensive testing of each lot minimizes contamination risks to your lab. Our MEF are derived and comprehensively tested on mouse and human ES cells to ensure robust and consistent performance with every lot. In addition they are:
- Negative for bacterial/fungal contamination
- Negative for Mycoplasma – PCR assay
- Negative for mouse pathogens
- Post thaw viability: typically greater than 95%
Recommended Plating
There is a wide range of MEF plating densities used by researchers for mouse and human pluripotent stem cell culturing. The density depends on the ESC or iPSC lines you are culturing. We recommend that you use the feeder density previously used by the source lab from which you received the cell line; or the density determined through personal experience to be appropriate for your specific stem cell line. GlobalStem’s MEF have been successfully used to support undifferentiated pluripotent stem cells at densities ranging from 2 x 104-5.3 x 104 cells/cm2.
The MEF should be plated 24 hours prior to plating the ESC or IPC and can be used for 7–10 days.
If the feeder cells are too sparse, they may not maintain the pluripotent cells without differentiation, and the pluripotent cells may not attach well. If the MEF are too dense, the feeder layer may detach from the plate, and the culture will be lost.
Gelatin Coating not Required
Our MEFs do not require gelatin coating of the plates prior to use. Using gelatin coating does not benefit or inhibit the ability of the MEFs to support pluripotent stem cells. We recommend not using gelatin with our MEFs because it adds in an extra step and could be a source off variation in culturing the cells. The use of gelatin is up to the individual researcher.