hESC Gene Expression Reference Cl.4/D3
Highly characterized stem cells for use as a gene expression reference for stem cell research. The reference cells express representative markers of the undifferentiated state of pluripotent stem cells providing more accurate comparison across experiments.
Click to enlarge |
|
| Recommended use | Normalization and Standardization of hESC and iPSC experimental data |
| Product Unit | Cryopreserved vial |
| Size | 5 million cells/vial |
| Storage | Liquid Nitrogen |
| Quality Testing | Tested for Sterility, human pathogens, Mycoplasma, Post thaw viability; tested for high expression of set of markers indicative of the undifferentiated state of hESC and iPSC |
| Shipping | Dry ice |
Gene Expression Reference Cells (2102Ep)
GlobalStem provides highly characterized stem cells for use as a gene expression reference for stem cell research. The cells provided are from the human embryonal carcinoma cell line 2102Ep clone 4/D3. The reference cells express representative markers of the undifferentiated state of pluripotent stem cells, providing more accurate comparison across experiments. The cells are ready for use after thawing and provide an ideal external reference for characterizing pluripotent stem cells by gene expression analysis. For ease of use, we offer live cryopreserved cells for gene expression and other applications.
The untreated 2102Ep reference cells are a stable and rich source of the genes commonly used as markers of undifferentiated hESC. Cells also highly express miRNAs specific to hESC lines.
The importance of normalization in gene expression is exemplified in the graphs to the right. Gene expression profile was examined using an Illumina Bead-Array containing 24,131 full-length and splice-variant transcripts from the human RefSeq database. The Reference 2102Ep cells expressed a similar number of genes as compared with undifferentiated hESC lines.
