hESC Flow Cytometry Standard-5M
Highly characterized stem cells for use as a flow cytometry reference for stem cell research. The reference cells express representative markers of the undifferentiated state of pluripotent stem cells providing more accurate comparison across experiments.
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| Recommended use | Normalization and Standardization of hESC and iPSC experimental data |
| Product Unit | Frozen vial |
| Size | 5 million cells/vial |
| Storage | -80°C |
| Quality Testing | Tested for Sterility, human pathogens, Mycoplasma; tested for high expression of set of markers indicative of the undifferentiated state of hESC and iPSC |
| Shipping | Dry ice |
Flow Cytometry Reference Standard (2102Ep ) Clone Cl.4/D3
GlobalStem provides highly characterized stem cells for use as a flow cytometry reference for stem cell research. Cells are fixed and from the human embryonal carcinoma cell line 2102Ep clone 4/D3. The reference cells express representative markers of the undifferentiated state of pluripotent stem cells, providing more accurate comparison across experiments. The cells are ready for use after thawing and provide an ideal external reference for characterizing pluripotent stem cells by flow cytometry analysis.
Paraformaldehyde fixed, nullipotent human embryonal carcinoma line provides standard measurements for undifferentiated pluripotent cells (iPS and ES). They are easily maintained as a monolayer in an undifferentiated, homogeneous population. ESC reference standard cells are analyzed for multiple markers of pluripotency and differentiation using flow cytometer.
Figure 1. Flow cytometry was used to analyze a bank of human ESC (HUES-22) established on mouse embryonic fibroblasts (GlobalStem GSC-6001G) and the data was compared to the standardized human embryonal carcinoma cell line 2102Ep (GlobalStem GSC-2102S). It can be observed that the banked HUES-22 cells are uniformly undifferentiated.
